Self-Integrating Multifunctional Amyloidogenic Protein-Fenugreek Composite Hydrogel Patch and Ointment for...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Wound Healing Research

Background:

• Deep muscle wound healing faces challenges like infection and slow regeneration.
• Conventional dressings have limitations including poor adherence and frequent replacement needs.

Purpose of the Study:

• To develop a self-adhering multifunctional composite hydrogel for deep muscle wound healing.
• To integrate bovine serum albumin (BSA) fibrils with fenugreek extract (FE) for enhanced therapeutic properties.

Main Methods:

• Engineered two topical formulations: a mechanically robust patch and a spreadable ointment.
• Characterized hydrogel physicochemical properties, including microstructure, mechanical strength, and rheology.
• Evaluated antioxidant, antibacterial, and biocompatibility profiles.
• Assessed efficacy in a rabbit full-thickness muscle wound model.

Main Results:

• Hydrogels exhibited porous microstructures, good moisture retention, and tunable viscoelastic properties.
• The patch showed superior mechanical strength and adhesion; the ointment offered facile application.
• Both formulations demonstrated significant free radical scavenging (approx. 87%), potent antibacterial activity, and excellent cell viability (>95%).
• Accelerated wound closure and epithelialization by day 21 in vivo, with reduced fibrosis and organized collagen.

Conclusions:

• The developed hydrogel system offers a promising therapeutic approach for complex musculoskeletal wounds.
• The self-adhering patch eliminates the need for secondary dressings, improving patient compliance.
• This multifunctional platform has potential for clinical translation in advanced wound care.