Related Experiment Video
Updated: Jan 15, 2026

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
Published on: September 10, 2015
Evolutionarily Diverged TRAPP Components in Giardia lamblia That Lacks a Classical Golgi
Avishikta Chatterjee1, Kuladip Jana1, Sandipan Ganguly2
1Department of Biological Sciences, Bose Institute, Kolkata, West Bengal, India.
Abstract:
The endomembrane system of the intestinal pathogen Giardia lamblia lacks a separate Golgi compartment. Without this sorting compartment, how cargo sorting to various subcellular destinations occurs within Giardia remains an open question. While the distribution of various Golgi-associated SNAREs and Rabs has been documented in this parasite, the TRAPP (TRAnsport Protein Particle) complex, a guanine nucleotide exchange factor for Golgi-associated Rabs, remained uncharacterized. Herein, we report that Giardia expresses a minimal set of TRAPP complex components, GlBet3, GlBet5, GlTrs23, and GlTrs31. Some of these components can interact with GlRab1a, GlRab11, and the COPII coat protein, GlSec23. Coupled with the colocalization and coimmunoprecipitation of GlBet3 and GlBet5, we propose the existence of a functional TRAPP complex in Giardia with an architecture that is different from that of yeast. While some interactions within this complex may be analogous to those in yeast, we find evidence of some unique interactions as well. The TRAPP genes are upregulated during encystation, and two components are associated with encystation-specific vesicles. Besides the endomembrane system, the presence of GlBet3 and GlBet5 at the plasma membrane, membrane wrapping ventral disc periphery, and the median body indicates that the TRAPP complex may support unique features and functions of Giardia.
More Related Videos
13:08Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
Published on: August 10, 2017
12:15The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy
Published on: October 3, 2017
Related Concept Videos
Diversity of Protists I
Cotranslational Protein Translocation
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Golgi Matrix Proteins
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
Golgi Apparatus
Golgi Apparatus
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Transport Across the Golgi