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Published on: March 11, 2020
A R2R3-MYB transcription factor SmMYB88 of Solanum melongena regulates cold tolerance by activating antioxidant
Yanhong Lan1,2,3, Fangyi Gong1,2,3, Rongjiang Dong1,2,3
1Key Laboratory of Germplasm Innovation and Utilization of Horticulture Crops of Sichuan Province, Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.
Abstract:
MYB transcription factors play a pivotal role in plant responses to cold stress. However, the underlying regulatory mechanisms in eggplant (Solanum melongena) remain unclear. In our previous study, based on transcriptomic analysis, we identified a MYB transcription factor as a core regulatory gene involved in cold stress responses in eggplant. In this study, we isolated and cloned SmMYB88 from eggplant. SmMYB88 was localized to the nucleus and exhibited transactivation activity. Heterologous overexpression of SmMYB88 in Arabidopsis thaliana enhanced the plant's cold tolerance. SmMYB88 specifically bound to the promote elements of SmGST and SmGPX. Furthermore, the interaction between SmMYB88 and SmERF1 further up-regulated the expression of SmGST and SmGPX genes. The up-regulated SmGST and SmGPX enhanced the plant's capacity to scavenge reactive oxygen species (ROS) by increasing glutathione metabolism and corresponding enzyme activities, thereby improving the plant's tolerance to cold stress. Additionally, overexpression of SmMYB88 significantly increased the expression levels of ICE1, CBFs, and COR47 in Arabidopsis (p < 0.05), suggesting that SmMYB88 may enhance plant cold tolerance by regulating the expression of CBF network genes. Therefore, this gene may serve as a potential target for genetic engineering to improve cold tolerance in eggplant.
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