Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

57.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.5K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.7K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.7K
What is Genetic Engineering?00:49

What is Genetic Engineering?

79.6K
Overview
79.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

21.4K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
21.4K
Homologous Recombination02:31

Homologous Recombination

62.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Caecal Bascule Volvulus: Diagnostic Challenges in a Rare cause of Large Bowel Obstruction.

Case reports in surgery·2026
Same author

A Biomarker Out of Context: Understanding High p-tau217 in the Developing Brain.

Molecular neurobiology·2026
Same author

Dynamic Carboxylic Acid Arms Enable Proton Shuttling in Iron-Based Hydrogen Catalysis.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Genome Editing of a Carotenogenic Gene for Lycopene Enhancement Increases Heavy Metal Stress Susceptibility in Tomato (Solanum lycopersicum L.).

Physiologia plantarum·2026
Same author

Internal herniation secondary to congenital mesenteric defect with associated fibrous congenital bands in an adult male.

Journal of surgical case reports·2026
Same author

Role of beneficial metalloids, silicon and selenium, in enhancing yield and quality in floricultural crops.

Plant science : an international journal of experimental plant biology·2026

Related Experiment Video

Updated: Jan 15, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K

Challenges and Opportunities with CRISPR-Based Genome Editing in Legume Crops.

Pawan Kumar1, Himanshu Yadav2, Badal Mahakalkar1

  • 1Department of Biotechnology, Central University of Haryana, Mahendergarh, 123029, Haryana, India.

Functional & Integrative Genomics
|January 14, 2026
PubMed
Summary

Genome editing technologies like CRISPR/Cas9 offer new ways to improve legume crops by addressing challenges in transformation and tissue culture. These advanced tools, including AI, accelerate the development of resilient and high-yielding legumes for sustainable agriculture.

Keywords:
Genome editing approachesMultiplex genome editingPrecision breedingStress toleranceTranslational genomes

More Related Videos

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

18.6K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

5.0K

Related Experiment Videos

Last Updated: Jan 15, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

18.6K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

5.0K

Area of Science:

  • Agricultural Science
  • Genomics
  • Molecular Biology

Background:

  • Significant advancements in legume genomics have occurred.
  • A gap exists between knowledge generation and its application in crop improvement.
  • Genome editing presents a powerful opportunity to bridge this gap.

Purpose of the Study:

  • To review recent developments in CRISPR/Cas9-based genome editing for legume crops.
  • To highlight applications of various genome editing techniques and their impact on crop improvement.
  • To discuss challenges and future directions in legume genome editing.

Main Methods:

  • Review of CRISPR/Cas9 variants (Cas variants/orthologs, PAMless editing, multiplex editing, base editing, prime editing, transcriptional regulation, methylome editing, DNA-free editing).
  • Discussion of repair pathways (NHEJ and HDR) for precise genomic modifications.
  • Exploration of virus-mediated editing, in planta transformation, mobile guide RNAs, and AI in genome editing.

Main Results:

  • CRISPR/Cas9 has been successfully applied to develop legumes with enhanced stress tolerance, improved architecture, nutrient uptake, yield, and quality.
  • Artificial intelligence aids in guide RNA design, off-target prediction, and novel Cas variant development.
  • Overcoming recalcitrance in transformation and tissue culture is crucial for widespread genome editing application in legumes.

Conclusions:

  • Genome editing offers significant potential for accelerating legume crop improvement.
  • Addressing technical limitations like recalcitrance is key to realizing this potential.
  • Integrating advanced genome editing tools with translational breeding is vital for sustainable agriculture and food security.