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Updated: Jan 17, 2026

Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
Tomato lateral root branching requires Nuclear Architecture Related 1 via auxin signaling, a process involving
Yueqiao Wang1, Pengfei Cheng1, Ke Jiang1
1Laboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Abstract:
The endogenous hydrogen (H2) synthesis pathway in higher plants has been a mystery for nearly a century. Here, we report that the tomato Nuclear Architecture Related 1 (NAR1) protein is an enzymatic source of plant-based H2. Mutation experiments found that the H2-producing hydrogenase activity is closely related to four H-cluster coordinating cysteine residues and is positively regulated in darkness. Contrary changes in H2 production and lateral root (LR) branching were individually observed in SlNAR1 overexpression and mutant lines. The homozygous lethal effect of knocking out tomato NAR1 was also observed. Genetic and anatomic evidence confirmed that NAR1 control of endogenous H2 is a signaling mechanism that manipulates the expression of polar auxin transporters. Auxin accumulation in LR primordium and oscillation zone and LR branching stimulation were subsequently observed. Pot-cultured transgenic tomato plants overexpressing SlNAR1 display a luxuriant root system. These findings identify a mechanism for genetic engineering to regulate root organogenesis through alteration of NAR1-dependent H2.
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