Mapping the visual cortex with Zebra noise and wavelets

Studies of the early visual system often require characterizing the visual preferences of large populations of neurons. This task typically requires multiple stimuli such as sparse noise and drifting gratings, each of which probes only a limited set of visual features. Here, we introduce a new dynamic stimulus with sharp-edged stripes that we term Zebra noise and a new analysis model based on wavelets, and we show that in combination they are highly efficient for mapping multiple aspects of the visual preferences of thousands of neurons. We used two-photon calcium imaging to record the activity of neurons in the mouse visual cortex. Zebra noise elicited strong responses that were more repeatable than those evoked by traditional stimuli. The wavelet-based model captured the repeatable aspects of the resulting responses, providing measures of neuronal tuning for multiple stimulus features: position, orientation, size, spatial frequency, drift rate, and direction. The method proved efficient, requiring only 5 minutes of stimulus (repeated three times) to characterize the tuning of thousands of neurons across visual areas. In combination, the Zebra noise stimulus and the wavelet-based model provide a broadly applicable toolkit for the rapid characterization of visual representations, promising to accelerate future studies of visual function.