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Updated: Jan 17, 2026

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
Published on: March 17, 2023
Covalently engineered PQDs-fluorescent protein FRET probes for tumor imaging and diagnosis
Jingli Bi1, Lingyun Gao1, Yufei Xu1
1School of Pharmacy, Jiangsu University, Zhenjiang, 212013, PR China.
Abstract:
Förster Resonance Energy Transfer (FRET)-based biosensors are powerful tools for monitoring biomolecular interactions and microenvironmental dynamics with high precision. Herein, we constructed two distinct FRET probes by covalently assembling fluorescent proteins (mCherry or sfGFP) onto CsPbX3 PQDs via enzyme-inhibitor-linked surface modification. The FRET probes respond to glutathione (GSH)-mediated reduction through disulfide bond cleavage, leading to modulations in energy transfer efficiency. Upon anti-HER2-mediated internalization, the PQDs-fluorescent protein probes facilitate real-time visualization of dynamic intracellular GSH fluctuations in living cells. The different GSH levels observed between malignant and normal cells serve as a critical biomarker for cancer detection, highlighting the potential of GSH-responsive nanoprobes for integrated diagnostic and therapeutic applications in oncology.
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