Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.6K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.6K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

5.4K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transferable Deep Reinforcement Learning With Edge-Contour-Depth Fusion for Autonomous Wireless Capsule Endoscopy Navigation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Convergence is not correctness: context-dependent performance of enhanced-sampling methods across biological complexity.

Nature communications·2026
Same author

Use of AI agents to assess preoperative frailty in cancer patients.

npj digital surgery·2026
Same author

Costimulatory biomarker-specific CD28 PET for early prediction of the response to PD-1 blockade in lung cancer.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Pulmonary ground glass opacity tumor: a curable subtype of lung cancer when treated within the "surgical curative time window".

Translational lung cancer research·2026
Same author

Simplified perioperative serplulimab and chemotherapy for resectable squamous NSCLC: a phase II trial with biomarker analysis.

Journal for immunotherapy of cancer·2026

Related Experiment Video

Updated: Jan 16, 2026

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

2.5K

Simulating Freely Diffusing Single-Molecule FRET Data with Consideration of Protein Conformational Dynamics.

James Losey1, Michael Jauch2, Axel Cortes-Cubero3

  • 1Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, United States.

The Journal of Physical Chemistry. B
|January 15, 2026
PubMed
Summary

This study introduces a new method to simulate single-molecule Förster resonance energy transfer (smFRET) data for flexible biomolecules. The simulation uses Langevin dynamics to generate realistic data for developing advanced smFRET analysis techniques.

More Related Videos

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
09:17

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

Published on: March 1, 2022

3.5K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

5.2K

Related Experiment Videos

Last Updated: Jan 16, 2026

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

2.5K
Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
09:17

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

Published on: March 1, 2022

3.5K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

5.2K

Area of Science:

  • Biophysics
  • Computational Biology
  • Biochemistry

Background:

  • Single-molecule Förster resonance energy transfer (smFRET) is crucial for studying biomolecular conformational dynamics.
  • Accurate simulation of smFRET data is vital for developing and validating analysis methods.

Purpose of the Study:

  • To develop a framework for generating high-fidelity simulated smFRET data for flexible biomolecules.
  • To enable the testing and refinement of smFRET data analysis techniques.

Main Methods:

  • Utilized Langevin dynamics to model conformational flexibility and generate interdye distance distributions.
  • Simulated freely diffusing smFRET timestamp data incorporating molecular diffusion and photon statistics.
  • Integrated molecular dynamics (MD) simulations for enhanced realism in data generation.

Main Results:

  • Successfully generated realistic smFRET timestamp data for conformationally flexible systems.
  • Demonstrated the integration of conformational dynamics, diffusion, and photon statistics in simulations.
  • Validated the developed module by comparing smFRET data analysis techniques.

Conclusions:

  • The Langevin dynamics module provides a robust framework for simulating smFRET data from systems with known conformational heterogeneity.
  • This approach facilitates the development of novel analysis techniques for flexible proteins and biomolecules.
  • The framework supports the creation of more realistic simulated smFRET data for advanced research.