Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Thickness-Dependent Semiconductor-Metal Transition in Two-Dimensional Nonlayered Magnetic CuCo<sub>2</sub>S<sub>4</sub>.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Transparent EMI shielding and a thermal insulating optical window based on a randomized metallic mesh and ITO-based coating.

Applied optics·2026

Same author

Malignant Melanoma Bone Metastasis With Unknown Primary Site: A Case Report and Literature Review.

Case reports in orthopedics·2026

Same author

Adhesion and environmental durability of anti-reflective coatings on cycloolefin copolymer.

Applied optics·2026

Same author

Molecular Sieve Promoted Growth of Ferroelectric Trilayer 3R-MoS<sub>2</sub> for Polarization-Dependent Reconfigurable Optoelectronic Synapses.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Femtosecond laser conditioning to enhance the ultraviolet laser-induced damage resistance of HfO<sub>2</sub> thin films.

Optics letters·2026

Same journal

Multi-tissue Metabolic GWAS and Drought-Responsive Multi-omics Reveal the Genetic Basis of the Quinoa Metabolome.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Bioinspired Multifunctional Flexible C-SiC Fibrous Aerogel for Superior Electromagnetic Interference Shielding Under Extreme Environments.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

RHINO: An Integrative Multi-Omics Framework Linking Circadian Physiology to Precision Medicine.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

From Chatbots to Co-Scientists: The Impact of Knowledge-Generating AI (AI 4.0) on Healthcare and Research.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Cobalt Single-Atom Nanozyme for Enhanced Intestinal Radioprotection and Tumor Radiosensitization via Bidirectional ROS Modulation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Ultrafast Optoacoustics Reveals Intricate 3D Anisotropic Elasticity in Nanocrystalline Membranes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 16, 2026

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Real-Time High-Definition Hyperspectral Endoscopy via Spatial-Temporal Low-Frequency-Stochastic Spectral Encoding.

Xiaowei Liu¹, Jiakang Shao², Julin Xiao^1,3,4

¹Research Centre for Frontier Fundamental Studies, Zhejiang Lab, Hangzhou, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|January 15, 2026

Summary

This summary is machine-generated.

This study introduces a novel hyperspectral endoscopy technique using spatial-temporal spectral encoding and an attention network. It achieves real-time imaging for improved in vivo diagnostics and monitoring during minimally invasive procedures.

Keywords:

diffuse reflectance hyperspectral endoscopy low‐frequency stochastic filter neural network spectrum encoding

More Related Videos

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Related Experiment Videos

Last Updated: Jan 16, 2026

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Area of Science:

Biomedical optics
Medical imaging
Endoscopy

Background:

Hyperspectral endoscopy offers detailed in vivo visualization but is limited by low frame rates.
Low frame rates hinder the capture of moving tissues, impacting diagnostics and monitoring during procedures.

Purpose of the Study:

To develop a low-latency hyperspectral endoscopy system for real-time in vivo imaging.
To overcome motion artifacts and enable high-resolution spectral analysis of tissues.

Main Methods:

Implemented a spatial-temporal spectral encoding approach with low-frequency stochastic filters.
Utilized an encoding-guided spectral attention network (ESANet) for hyperspectral image reconstruction.
Developed a prototype system achieving 20 Hz frame rate and 67 spectral channels (420-750 nm).

Main Results:

Achieved real-time frame rate (20 Hz) and high-definition resolution, overcoming tissue motion.
Captured fine superficial features, including capillaries as small as 37 µm.
Visualized distinct spectral characteristics and rapid compositional changes in photodynamic therapy and hepatic ischemia models.

Conclusions:

The proposed hyperspectral endoscopy system provides a cost-efficient and adaptable solution.
Enables enhanced in vivo diagnostics, efficacy assessment, and risk monitoring.
Has broad applicability for various medical imaging scenarios.