Comparison study of microfluidic sperm selection chips with density gradient centrifugation and upstream methods in...

This study compared microfluidic sperm selection chips with density gradient centrifugation and swim-up methods using 60 semen samples. All techniques improved sperm motility and reduced DNA fragmentation, abnormal morphology, and leukocyte levels. The microfluidic chip yielded sperm with the best motility, morphology, and DNA integrity, though with a lower recovery rate and concentration than traditional methods. Progressive motility recovery rates were similar across groups. Despite lower sperm yield, microfluidic chips offer a promising option for selecting high-quality sperm in fertility treatments.