Cellulomonas olei sp. nov., isolated from oil-contaminated soil

A bacterial strain, designated as P4^T, was isolated from an oil-contaminated soil sample. The cells were Gram-stain-positive, short rod-shaped and motile with a single lateral flagellum. Growth of the strain was observed at 20-40 °C (optimum at 30 °C), at pH 6.0-11.0 (optimum at pH 7.0) and with 0-5.0 % (w/v) NaCl (optimum at 1.0%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P4^T was closely related to the members of genus Cellulomonas, with the highest sequence similarity to Cellulomonas hominis DSM 9581^T (99.5%), followed by Cellulomonas endometrii Marseille-Q7820^T (99.1%), Cellulomonas pakistanensis NBRC 110800^T (99.0%) and Cellulomonas denverensis NBRC 110783^T (98.1%). The draft genome sequence of strain P4^T was 3.98 Mb in length, with a DNA G+C content of 75.5 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain P4^T and the closely related type strains were in the range of 78.6-84.4 % and 21.9-28.2 %, respectively. The predominant cellular fatty acids of strain P4^T included anteiso-C_15 :0, C_16:0, C_14:0, iso-C_15:0 and anteiso-C_17:0. The main polar lipid was composed of diphosphatidylglycerol, glycophospholipid, glycolipid and three phosphatidylinositol mannosides and the major menaquinone was MK-9(H4). Based on the polyphasic characterization, strain P4^T (=KCTC 49393^T=CCTCC AB 2019402^T) represents a novel species of the genus Cellulomonas, for which the name Cellulomonas olei sp. nov. is proposed.