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Near-Infrared Cocrystal Nanofluorophore with Enhanced Two-Photon Absorption Cross Sections.

Liangmeng Hao1, Ying Ni1, Jiawei Huang2

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Researchers developed new organic nanoparticles (BP4TC-NPs) for advanced biological imaging. These nanoparticles efficiently absorb near-infrared light for deep red fluorescence, offering a promising tool for multiphoton imaging with low cytotoxicity.

Keywords:
cocrystalimagingnanoparticletheorytwo‐photon absorption

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biophotonics

Background:

  • Organic nanoprobes with broadband two-photon absorption (TPA) and near-infrared (NIR) excitation are crucial for biological imaging and photonics.
  • Designing and preparing such materials presents significant challenges.

Purpose of the Study:

  • To develop a novel organic nanoprobe material with broadband TPA and NIR optical excitation.
  • To characterize the TPA properties and biological applicability of the synthesized material.

Main Methods:

  • Co-crystallization strategy to synthesize BP4TC (donor BP4VA, acceptor TCNB).
  • Nanoprecipitation to create water-dispersible BP4TC nanoparticles (BP4TC-NPs).
  • Open-aperture Z-scan measurements for TPA cross-section determination.
  • First-principles calculations and TDDFT approach for theoretical validation.
  • In-cell imaging using A549 cells (human lung cancer cells).

Main Results:

  • BP4TC-NPs exhibit broadband TPA characteristics from 700-1000 nm with decreasing TPA cross-sections.
  • First experimental determination of TPA cross-section for a molecular multicomponent solid.
  • First-principles calculations confirm enhanced intermolecular charge transfer and TPA ability.
  • Bright, low-background fluorescence observed in A549 cells under 900 nm excitation.
  • Negligible cytotoxicity demonstrated for BP4TC-NPs.

Conclusions:

  • BP4TC-NPs represent a potent two-photon absorber with broadband NIR-I TPA and favorable biological properties.
  • The developed cocrystal material serves as a multifunctional platform for biological multiphoton imaging and NIR photonics.
  • Provides a validated blueprint for designing advanced nonlinear optical nanomaterials.