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Updated: Jan 18, 2026
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Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
Published on: December 3, 2019
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Oyster cellular architecture and innate immune programs resolved by single-cell RNA sequencing.
1School of Energy and Environment and State Key Laboratory of Marine Environmental Health, City University of Hong Kong, Kowloon, Hong Kong, China; Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China.
Fish & Shellfish Immunology
|January 15, 2026
Summary
This study maps cell types in oyster mucosal tissues, revealing specialized immune cells and neural-epithelial communication critical for invertebrate health in changing coastal environments.
Area of Science:
- Marine invertebrate immunology
- Single-cell genomics
- Innate immunity
Background:
- Barrier mucosal tissues are vital for marine invertebrate filtration, gas exchange, and immunity.
- The cellular organization and functional division within these tissues are poorly understood.
Purpose of the Study:
- To create a tissue-resolved single-cell atlas of Crassostrea hongkongensis.
- To define cell types and their immune functions.
- To elucidate communication networks within mucosal barrier tissues.
Main Methods:
- Single-cell RNA sequencing
- Tissue-resolved atlas generation
- Ligand receptor inference
Main Results:
- Defined twenty cell types, including a diversified phagocyte lineage with distinct immune strategies.
- Identified three humoral immune populations collaborating with phagocytes and epithelial cells.
- Revealed neural-epithelial communication via NCAM and PTPRM pathways regulating immune activity.
Conclusions:
- Established a cellular taxonomy for mapping immune programs in specific contexts.
- Demonstrated the role of barrier epithelium and neural cells in coordinating immune responses.
- Advanced understanding of marine invertebrate resilience in variable coastal environments.
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