Related Experiment Video

Updated: Jan 18, 2026

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

3.1K

E2F1 K117 methylation by SETD6 disrupts BRD4-E2F1 binding and modulates E2F1 chromatin binding and gene regulation in

Gizem Tugce Ulu1, Margarita Kublanovsky2,3, Raz Shalev2,3

  • 1Institute of Biochemistry, University of Stuttgart, Allmandring 31, Stuttgart 70569, Germany.

Nucleic Acids Research
|January 16, 2026
PubMed

Insights

SETD6 protein lysine methyltransferase monomethylates transcription factor E2F1, impacting gene regulation and oncogenic phenotypes in prostate cancer. This methylation switch controls E2F1

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cancer Research

Background:

  • SETD6 (SET domain-containing protein 6) is a lysine methyltransferase involved in cellular processes, including cancer.
  • SETD6 monomethylates the transcription factor E2F1, but the functional outcomes remain largely unknown.
  • E2F1 plays a critical role in cell cycle regulation and cancer development.

Purpose of the Study:

  • To investigate the role of SETD6-mediated E2F1 K117 monomethylation in prostate cancer.
  • To elucidate the mechanism by which SETD6 methylation affects E2F1 function and chromatin interaction.
  • To determine the impact of E2F1 methylation on oncogenic phenotypes.

Main Methods:

  • Chromatin binding assays
  • Gene expression analysis
  • Biochemical and cellular assays
  • Genomic assays

Main Results:

  • SETD6-mediated E2F1 methylation alters E2F1's chromatin binding and gene upregulation profiles.
  • E2F1 methylation by SETD6 influences oncogenic phenotypes in prostate cancer cells.
  • SETD6-mediated K117 methylation prevents E2F1 K117 acetylation, regulating E2F1-BRD4 interaction.

Conclusions:

  • SETD6-mediated K117 methylation acts as a molecular switch, controlling E2F1's interaction with BRD4 via a methylation/acetylation mechanism.
  • This regulatory switch modulates E2F1's chromatin binding and downstream gene expression, affecting cellular phenotypes.
  • The findings suggest a broader role for SETD6 in regulating transcription factor activity through similar methylation/acetylation mechanisms.

More Related Videos

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.3K
Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

8.8K

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.7K
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.5K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.3K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.3K