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Updated: Jan 18, 2026

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

Published on: October 23, 2019

B-Cell Chronic Lymphocytic Leukemia in a Cat.

James Andre Mori¹, Emma Stacey¹, Yelda El Helou²

¹Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.

Veterinary Clinical Pathology

|January 16, 2026

Summary

A Persian cat diagnosed with B-cell chronic lymphocytic leukemia (B-CLL) showed symptom resolution and reduced leukocytosis after treatment with prednisolone and chlorambucil.

Keywords:

PARR feline flow cytometry gammopathy lymphocytosis polymerase chain reaction for antigen receptor rearrangement serum protein electrophoresis

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Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Published on: November 26, 2018

Area of Science:

Veterinary Oncology
Hematology
Immunology

Background:

A Persian cat presented with lethargy, hyporexia, weight loss, pale mucous membranes, enlarged lymph nodes, and splenomegaly.
Complete blood count revealed extreme leukocytosis (lymphocytes) and microcytic anemia.

Purpose of the Study:

Diagnose the cause of extreme leukocytosis in a feline patient.
Classify circulating leukocytes.
Evaluate treatment response for feline leukemia.

Main Methods:

Blood film review, biochemical and iron analysis, abdominal ultrasound.
Flow cytometry for leukocyte classification.
Polymerase chain reaction for antigen receptor rearrangement (PARR) and protein electrophoresis.

Main Results:

Flow cytometry identified lymphocytes as B-cell lineage (CD18+, CD21+, MHC II+).
PARR confirmed clonal rearrangement (IGH2, IGH3 loci).
Treatment with prednisolone and chlorambucil led to clinical improvement and decreased leukocytosis.

Conclusions:

The cat was diagnosed with B-cell chronic lymphocytic leukemia (B-CLL), a rare feline leukemia.
Favorable response to alkylating therapy was observed.
The cat survived for 19 months post-diagnosis.