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Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
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Developing bacterial resistance to antibiotics: a laboratory activity.

Maya Hagander1, Claire DeWeese1, Rachel Boyette1

  • 1Department of Chemistry, Wittenberg University, Springfield, OH 45504, USA.

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|January 16, 2026
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Summary

This study introduces a simple lab activity for undergraduates to observe bacterial antibiotic resistance development within a week. Students witness how easily bacteria become resistant, reinforcing natural selection concepts.

Keywords:
antibiotic resistancelaboratory activityscience awareness

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Antibiotic resistance is a significant global health threat.
  • Educational initiatives are crucial for awareness and combating resistance.
  • Undergraduate laboratory experiences can effectively demonstrate resistance mechanisms.

Purpose of the Study:

  • To develop and present a simplified laboratory activity for undergraduates.
  • To demonstrate the rapid development of bacterial antibiotic resistance.
  • To enhance student understanding of natural selection and antibiotic resistance.

Main Methods:

  • A week-long laboratory procedure in a single container to observe bacterial resistance.
  • Variations exploring antibiotic concentration and additivity.
  • Students perform basic microbiology techniques, including culture growth and Minimum Inhibitory Concentration (MIC) determination.

Main Results:

  • Reliable generation of resistant bacterial strains was achieved.
  • Commonly observed eightfold increases in bacterial resistance levels.
  • Students gained practical microbiology skills and observed resistance development firsthand.

Conclusions:

  • The laboratory activity effectively illustrates the ease of bacterial resistance development.
  • The activity connects empirical observation to the principles of natural selection.
  • This educational tool promotes critical understanding and awareness of antibiotic resistance.