Astrocyte to neuron reprogramming with NeuroD1 for repair in canine stroke
Isaac H Clark1,2,3, Zachary Roushdy2, Dilmareth Natera-Rodriguez2,3
1Biomedical Engineering Graduate Program, University of Minnesota, Minneapolis, MN, United States.
Frontiers in Stroke
|January 16, 2026
Summary
This study explored reprogramming astrocytes into neurons to treat ischemic stroke in dogs. Treated dogs showed improved functional recovery and reduced brain damage, suggesting a promising therapeutic avenue.
Area of Science:
- Neuroscience
- Regenerative Medicine
- Stroke Research
Background:
- Stroke causes significant neuronal damage and long-term disability.
- Current treatments for ischemic stroke have limitations.
- Astrocyte-to-neuron reprogramming is an emerging therapeutic strategy.
Purpose of the Study:
- To investigate astrocyte-to-neuron reprogramming for ischemic stroke treatment.
- To assess anatomical and functional recovery in a canine model.
- To evaluate the efficacy of NeuroD1-mediated reprogramming.
Main Methods:
- Utilized an adeno-associated viral (AAV) vector to deliver NeuroD1 to astrocytes.
- Employed MRI for anatomical analysis and neurological severity scoring for behavioral assessment.
- Conducted immunohistochemistry to analyze cellular changes, including astrocyte and microglial activation.
Main Results:
- Treated animals demonstrated faster and more extensive behavioral recovery compared to controls.
- Anatomical analysis revealed reduced ventricle enlargement in treated animals post-stroke.
- Cellular analysis indicated decreased astrocyte and microglial activation in treated subjects.
Conclusions:
- NeuroD1-mediated astrocyte reprogramming shows potential in reducing neuroinflammation.
- This approach may enhance functional recovery following ischemic stroke.
- Further research is warranted to explore this therapeutic strategy.
