A guide to selecting high-performing antibodies for GCase (UniProt ID: P04062) for use in western blot,
Donovan Worrall1, Charles Alende1, Maryam Fothouhi1
1Neuroscience and Neurosurgery, Montreal Neurological Institute-Hospital, Montreal, Québec, Canada.
Abstract:
The human GBA1 gene encodes glucocerebrosidase (GCase), a lysosomal enzyme that hydrolyzes glucosylceramides. Variants in GBA1 and reduced GCase activity have been linked to Parkinson's disease and Gaucher's disease. Here we have characterized twenty-four GCase commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.
