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Related Experiment Video

Updated: Jul 21, 2026

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
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Published on: July 21, 2020

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Mpox virus replicates in lung organoids without significantly affecting their cellular function.

Yoshitaka Nakata1, Keiya Uriu2, Rina Hashimoto1

  • 1Department of Synthetic Human Body System, Medical Research Laboratory, Institute of Science Tokyo, Tokyo, 113-8510, Japan.

Biochemistry and Biophysics Reports
|January 16, 2026
PubMed
Summary

Mpox virus (MPXV) can replicate in lung organoids, but does not significantly impact cellular function or trigger a major inflammatory response. This suggests limited respiratory damage from MPXV infection.

Keywords:
Lung organoidsMpoxMpox virusiPS cells

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Area of Science:

  • Virology
  • Respiratory Medicine
  • Organoid Technology

Background:

  • Mpox virus (MPXV) infection can cause respiratory symptoms.
  • MPXV genome detected in throat swabs suggests potential respiratory tract involvement.
  • Understanding MPXV's effect on the respiratory system is crucial.

Purpose of the Study:

  • To investigate the effects of MPXV infection on lung organoids.
  • To evaluate viral replication and host response in the respiratory system.
  • To determine if MPXV infection alters lung organoid cellular function.

Main Methods:

  • Utilized human lung organoids as a model system.
  • Inoculated organoids with MPXV.
  • Assessed viral replication via genome quantification.
  • Analyzed histological changes using H&E staining.
  • Evaluated host response using RNA-sequencing and cytokine analysis.

Main Results:

  • High levels of MPXV genomes accumulated within lung organoid cells.
  • No significant histological differences observed between infected and uninfected organoids.
  • MPXV infection did not significantly alter lung marker gene expression.
  • No significant changes in the production of key proinflammatory cytokines (IL-1β, IL-6, TNF-α, IFN-β) were detected.

Conclusions:

  • MPXV replicates efficiently in lung organoids.
  • MPXV infection does not substantially impair lung organoid cellular function.
  • MPXV does not induce a significant proinflammatory cytokine response in this model.
  • Lung organoids serve as a valuable model for studying MPXV-respiratory tract interactions.