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Updated: Jan 18, 2026

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
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Atypical GPCR Activation Resolved by Nanobody Engineering

Roman R Schlimgen1,2, Shawn E Jenjak1, Alexa De La Sancha1

  • 1Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA.

Biorxiv : the Preprint Server for Biology
|January 16, 2026
PubMed
Summary

Researchers discovered a new way G protein-coupled receptors (GPCRs) activate, focusing on extracellular pocket volume changes instead of traditional conformational shifts. This finding offers novel strategies for targeting difficult-to-treat receptors.

Keywords:
ACKR3ChemokineCryo-EMGPCRNMRNanobodyVHH

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