Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Calorimetry01:19

Calorimetry

4.3K
When objects at different temperatures are placed in contact with each other but isolated from everything else, they attain thermal equilibrium. A container that prevents heat transfer in or out is called a calorimeter, and the use of a calorimeter to make measurements is called calorimetry. Generally, these measurements involve heat or specific heat capacity. The term "calorimetry problem" is used for any problem where the specified objects are thermally isolated from their...
4.3K
Constant Volume Calorimetry02:41

Constant Volume Calorimetry

30.5K
Calorimeters are useful to determine the heat released or absorbed by a chemical reaction. Coffee cup calorimeters are designed to operate at constant (atmospheric) pressure and are convenient to measure heat flow (or enthalpy change) accompanying processes that occur in solution at constant pressure. A different type of calorimeter that operates at constant volume, colloquially known as a bomb calorimeter, is used to measure the energy produced by reactions that yield large amounts of heat and...
30.5K
Constant Pressure Calorimetry03:02

Constant Pressure Calorimetry

97.2K
Calorimetry is a technique used to measure the amount of heat involved in a chemical or physical process or to measure the heat transferred to or from a substance. The heat is exchanged with a calibrated and insulated device called the calorimeter. Calorimetry experiments are based on the assumption that there is no heat exchange between the insulated calorimeter and the external environment. The well-insulated calorimeters prevent the transfer of heat between the calorimeter and its external...
97.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct Measurement of Adipose Thermogenesis by Isothermal Microcalorimetry.

Cells·2026
Same author

Thermogenesis is limited by cellular competence.

Frontiers in cell and developmental biology·2026
Same author

The Sleeping Wolf - Widefield OCT Angiography Revelations of Insidious Systemic Lupus Erythematous-Associated Retinal Vasculitis.

Retinal cases & brief reports·2026
Same author

Isoform-Specific Control of Adipose Thermogenesis by the miR-27 Family Reveals Antagonism within a Polycistronic miRNA Cluster.

bioRxiv : the preprint server for biology·2025
Same author

Exosome Trafficking Is a Key Regulator of Adipocyte Thermogenesis.

bioRxiv : the preprint server for biology·2025
Same author

Cytokines in PD-1 immune checkpoint inhibitor adverse events and implications for the treatment of uveitis.

BMC ophthalmology·2024

Related Experiment Video

Updated: Jan 18, 2026

Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans
04:54

Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans

Published on: June 2, 2023

2.0K

Quantifying Adipose Tissue Thermogenesis Using Highly Sensitive Isothermal Microcalorimetry.

Pauke C Schots, Devesh Kesharwani, Chad C Doucette

    Biorxiv : the Preprint Server for Biology
    |January 16, 2026
    PubMed
    Summary
    This summary is machine-generated.

    This study optimizes isothermal microcalorimetry for measuring adipose tissue thermogenesis. It provides practical guidance for experimental design and tissue handling to ensure accurate heat production measurements.

    More Related Videos

    Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity
    08:16

    Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity

    Published on: September 28, 2022

    2.8K
    Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
    08:31

    Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

    Published on: July 1, 2021

    3.5K

    Related Experiment Videos

    Last Updated: Jan 18, 2026

    Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans
    04:54

    Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans

    Published on: June 2, 2023

    2.0K
    Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity
    08:16

    Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity

    Published on: September 28, 2022

    2.8K
    Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
    08:31

    Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

    Published on: July 1, 2021

    3.5K

    Area of Science:

    • Metabolic Physiology
    • Biophysics

    Background:

    • Isothermal microcalorimetry (IMC) directly measures heat production, crucial for understanding thermogenesis.
    • Optimal IMC parameters for adipose tissue research remain undefined, limiting its application.

    Purpose of the Study:

    • To establish and validate experimental conditions for quantifying thermogenesis in adipose tissue using high-throughput IMC.
    • To provide practical guidelines for tissue handling, assay design, and data interpretation.

    Main Methods:

    • Utilized the CalScreener high-throughput IMC platform.
    • Assessed thermogenesis in adipocyte organoids, isolated adipocytes, and adipose tissue explants.
    • Investigated the impact of tissue handling and storage on thermogenic capacity.

    Main Results:

    • Heat output in organoids scaled with size and number, enhancing measurement consistency.
    • Freshly isolated adipocytes and intact explants showed robust, depot-specific thermogenesis.
    • Intact adipose tissue maintained ex vivo thermogenic capacity for 6 hours in nutrient medium.
    • Cold storage conditions, while preserving mitochondrial integrity, suppressed basal heat production.

    Conclusions:

    • Established optimized parameters for adipose tissue thermogenesis measurement via IMC.
    • Demonstrated the feasibility of batch analysis for large cohorts using ex vivo adipose tissue.
    • Highlighted the importance of appropriate tissue handling and storage for accurate thermogenic assessments.