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Liposome purification from micromolar protein background using diffusiophoretic trapping.

Pavlina Senikoglou1, Corentin Cardot1, Jonas N Pedersen1

  • 1Department of Health Technology, Technical University of Denmark, Kongens Lyngby, Denmark. rcwm@dtu.dk.

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Summary
This summary is machine-generated.

This study presents a fluidic device for purifying colloids like liposomes from liquid biopsies using diffusiophoresis. Pluronic polymer coating effectively prevents protein adsorption, enabling efficient extracellular vesicle biomarker isolation for early disease diagnostics.

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Area of Science:

  • Biomarker Discovery
  • Nanotechnology
  • Fluid Dynamics

Background:

  • Liquid biopsies offer a non-invasive method for early disease diagnostics using extracellular vesicle (EV) biomarkers.
  • Colloid concentration in fluidic devices, using diffusiophoresis and diffusioosmosis, is a key step for EV biomarker analysis.
  • Protein adsorption and surface interactions challenge colloid purification in complex biological samples like plasma.

Purpose of the Study:

  • To develop and validate a fluidic device for efficient colloid purification from liquid biopsies.
  • To investigate the role of surface modification in preventing protein adsorption and enabling diffusiophoretic trapping.
  • To demonstrate the purification of liposomes from plasma for potential application in early disease diagnostics.

Main Methods:

  • Utilized a fluidic device with a salt gradient to induce diffusiophoresis and diffusioosmosis for colloid trapping.
  • Employed Pluronic polymer coating for surface passivation to prevent protein adsorption and maintain surface zeta potential.
  • Experimentally captured and purified 120 nm liposomes from solutions containing bovine/human serum albumin and 10% plasma.

Main Results:

  • Pluronic coating effectively prevented adsorption of albumin and liposomes while maintaining a sufficient zeta potential for trapping.
  • Liposomes were successfully captured from dilute solutions with micromolar protein concentrations.
  • Demonstrated effective purification of liposomes from 10% plasma, with proteins removed via diffusion.

Conclusions:

  • Diffusiophoretic trapping, enhanced by Pluronic surface passivation, is a viable method for purifying extracellular vesicles from liquid biopsies.
  • This technique addresses challenges posed by protein-rich biological fluids, bringing colloid purification closer to clinical diagnostic applications.
  • The developed fluidic device shows promise for advancing early disease detection through improved biomarker isolation.