Related Experiment Video
Updated: Jan 18, 2026
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
A SERS-active microneedle for detecting oxidative capacity and redox potential of rats with high glucose-induced
Jiajia Wang1, Lingling Ji2, Yuyao Zhang3
1State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 211189, China; Department of Acupuncture-Moxibustion, Massage and Rehabilitation, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Abstract:
To detect oxidative capacity and redox potential of organism simultaneously, a surface-enhanced Raman scattering-active microneedle was fabricated by integrating oxidative capacity and redox potential surface-enhanced Raman scattering probes in two grooves on an acupuncture needle, respectively. Different redox pairs solutions were used to assess the detection feasibility of the surface-enhanced Raman scattering-active microneedles. Oxidative capacity surface-enhanced Raman scattering probes, based on p-phenylenediamine, can discriminate minor changes in oxidative capacity, and redox potential surface-enhanced Raman scattering probes, based on an anthraquinone derivative, distinguish changes in redox potential of different redox pairs. Rats with or without high glucose-induced stress were utilized to evaluate the detection feasibility of the surface-enhanced Raman scattering-active microneedles in vivo. In different tissues, the two parameters show slight discrepancies. In the muscles of rats with high glucose-induced stress, no correlation was observed among oxidative capacity, redox potential, and blood glucose levels. The surface-enhanced Raman scattering-active microneedles would be a versatile tool for researches of oxidative capacity and redox potential in physiological and pathological processes.
