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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Electrospray Ionization (ESI) Mass Spectrometry01:12

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
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Continuous Flow Paper Spray Ionization Mass Spectrometry for In-Depth Characterization of Anticancer Drugs in

Pallab Basuri1, Konrad Klinghammer2, Oliver Klein3

  • 1Department of Bioanalytical Chemistry, Institute of Chemistry, Humboldt-Universität zu Berlin, 12489 Berlin, Germany.

Journal of the American Society for Mass Spectrometry
|January 16, 2026
PubMed
Summary

Continuous flow paper spray ionization mass spectrometry (CFPSI MS) enables rapid detection of anticancer drugs in tissues. This method quantifies drug absorption, with palbociclib showing the highest bioabsorption in mouse models.

Keywords:
CFPSIanalysisanticancercontinuousdrugsflowmasspaperrapidsamples.spectraltissue

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Pharmacology

Background:

  • Accurate quantification of anticancer drugs in solid tissues is crucial for therapeutic drug monitoring.
  • Existing methods for drug analysis in tissues can be time-consuming and complex.
  • Understanding drug distribution and absorption within tissues is essential for optimizing treatment efficacy.

Purpose of the Study:

  • To introduce and validate a novel continuous flow paper spray ionization mass spectrometry (CFPSI MS) method.
  • To enable rapid detection and semiquantitative analysis of anticancer drugs in solid tissue samples.
  • To investigate the differential absorption and in-source chemical reactivity of specific anticancer drugs in a preclinical model.

Main Methods:

  • Development of a continuous flow paper spray ionization (CFPSI) technique.
  • Application of CFPSI MS for the analysis of palbociclib, copanlisib, and olaparib in patient-derived xenograft (PDX) mouse tissue samples.
  • Utilizing tandem mass spectrometry to explore drug reactivity and spectral complexity.

Main Results:

  • CFPSI MS successfully detected and characterized anticancer drugs in solid tissue samples.
  • Differential absorption of palbociclib, copanlisib, and olaparib was observed, with palbociclib exhibiting the highest bioabsorption.
  • Tandem mass spectrometry revealed significant spectral complexity due to in-source chemical reactivity of the drugs.

Conclusions:

  • CFPSI MS is a promising technique for rapid, semiquantitative analysis of anticancer drugs in tissues.
  • The study underscores the importance of spectral interpretation in complex biological matrices for accurate drug quantification.
  • Findings support the further development of CFPSI MS for clinical applications in therapeutic drug monitoring.