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Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Combinatorial Gene Control

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Related Experiment Video

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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

Temporal multiomics gene expression data across human embryonic stem cell-derived polyhormonal cell differentiation.

Abdurrahman Keskin¹, Hani J Shayya², Achchhe Patel³

¹Department of Biological Sciences, Columbia University, New York, NY, 10027, USA.

Scientific Data

|January 16, 2026

Summary

This study maps gene expression changes during human embryonic stem cell differentiation into pancreatic cells. The multi-omics dataset reveals key regulatory mechanisms in early development and lineage specification.

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Last Updated: Jul 5, 2026

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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

Developmental Biology
Stem Cell Biology
Genomics

Background:

Human embryonic stem cells (hESCs) are crucial for studying early human development and lineage specification.
Understanding the regulatory programs governing differentiation is essential for regenerative medicine and disease modeling.

Purpose of the Study:

To generate a high-resolution, temporal multi-omics dataset tracking molecular changes during hESC differentiation.
To investigate the dynamics of mRNA, translation, and protein expression during differentiation into definitive endoderm and polyhormonal cells.

Main Methods:

RNA-sequencing (RNA-seq) for transcriptome analysis.
Ribosome profiling for translational control assessment.
Quantitative mass spectrometry-based proteomics for protein abundance profiling.

Main Results:

Comprehensive temporal data on transcriptional, translational, and protein expression dynamics.
High technical quality with strong reproducibility across biological replicates.
Detailed characterization of molecular changes during endodermal and pancreatic lineage commitment.

Conclusions:

The dataset provides critical insights into regulatory mechanisms driving polyhormonal cell differentiation.
This resource enables deeper exploration of mammalian development and endodermal lineage specification.
Facilitates research on gene regulation during early human development.