Related Experiment Video
Updated: Jun 12, 2026
Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
Published on: March 12, 2022
Predicting progression to proliferative diabetic retinopathy using automated versus manual quantification of retinal
Aditya Verma1,2, Muneeswar G Nittala1, Roxan Mansoori Dara1,3
1Doheny Eye Institute, Pasadena, CA, USA.
Purpose:
To compare automated and manual quantification of retinal haemorrhages in eyes with diabetic retinopathy (DR) and to analyse the risk of progression to proliferative DR (PDR).
Methods:
Retinal haemorrhages on ultra-widefield (UWF) pseudocolor images in eyes with non-proliferative diabetic retinopathy (NPDR) were manually segmented. DR severity was assessed within the seven ETDRS fields at baseline and 1-year follow-up. Lesions were also automatically segmented using EyeRead UWF software (Eyenuk) and the frequency and area of retinal haemorrhages and the average distance of haemorrhages from the optic nerve centre were computed. Manual and automated results were compared and correlated with progression to PDR at one year.
Results:
Sixty-three eyes with NPDR at baseline were included, of which 29 progressed to PDR over one year. The automated measurements of total haemorrhage frequency, area and the distance from the optic nerve were significantly lower compared to manual grading, but the parameters were significantly correlated (r = 0.5-0.96; all P < 0.001). The distance of the haemorrhages from the optic nerve was found to be a significant risk factor for progression to proliferative DR from both approaches.
Conclusions:
Automated detection of retinal haemorrhages may serve as a surrogate to the manual grading in predicting the progression to PDR. Although the automated approach detects a lower number of lesions compared to manual grading, the results are correlated and the automatically determined parameters are still predictive of progression.
