Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Angiogenin alleviates lipotoxicity-induced impairment of skin wound healing.

Cellular & molecular biology letters·2026

Same author

Electrical Imaging of DNA Substructures Using Quasi-Static Nanopore Scanning.

Nano letters·2026

Same author

Recent Advances on the Chemical Compositions, Biological Activities, and Applications of Ziziphi Spinosae Semen: From Traditional Treasures to Modern Medical Stars.

Phytochemical analysis : PCA·2026

Same author

pAnno: a comprehensive, precise, and fast proteogenomic workflow for the discovery of novel coding regions.

Genome biology·2026

Same author

Construction of an Extracellular Matrix-related Gene Model for Predicting Prognosis and Immune Features in Gastric Cancer.

Current molecular medicine·2026

Same author

SERTAD4-AS1 suppresses pancreatic cancer progression by stabilizing SERTAD4 and inhibiting the Notch1 pathway.

Genes & diseases·2026

Search research articles

Related Experiment Video

Updated: Jan 18, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Azo-Enhanced Raman Rotors: Bridging Raman Scattering and Fluorescence for Hyperspectral Imaging of Live-Cell

Wanyi Xie¹, Yajun Yu², Yaping Peng¹

¹College of Chemistry, Central China Normal University,Wuhan, Hubei 430079, China.

Analytical Chemistry

|January 17, 2026

Summary

Azobenzene photoswitches offer dual Raman and fluorescence signals for hyperspectral imaging. This study uses their intensity ratio to accurately measure microviscosity in live cells, bridging spectral gaps for bioimaging.

More Related Videos

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Published on: August 31, 2022

Related Experiment Videos

Last Updated: Jan 18, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Published on: August 31, 2022

Area of Science:

Photochemistry
Spectroscopy
Biophysics

Background:

Azobenzene derivatives are photoswitches with tunable optical properties.
They can act as Raman reporters via azo-enhanced Raman scattering.
Simultaneous Raman and fluorescence measurements are challenging due to distinct spectral properties.

Purpose of the Study:

To develop a method for simultaneously measuring Raman scattering and fluorescence from azobenzene derivatives.
To leverage the fluorescence-to-Raman intensity ratio for microviscosity sensing in live cells.
To enable hyperspectral imaging of physicochemical heterogeneity in biological systems.

Main Methods:

Fine-tuning azo-enhanced Raman rotors for hyperspectral imaging.
Utilizing the fluorescence-versus-Raman intensity ratio as a self-calibrated readout.
Applying the method to visualize microviscosity changes in cellular organelles.

Main Results:

Demonstrated accurate visualization of microviscosity in live cell organelles (mitochondria, lysosomes, ER).
Successfully bridged the spectral gap between Raman scattering and fluorescence.
Established a robust method for probing physicochemical heterogeneity.

Conclusions:

The developed molecular platform enables simultaneous harnessing of Raman and fluorescence signals.
This approach offers new avenues for advanced hyperspectral bioimaging and cellular diagnostics.
The method provides a robust tool for mechanistic studies of heterogeneous biological and nonbiological interfaces.