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Updated: Jan 19, 2026
A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
Published on: February 19, 2016
Aptamer-regulated silver core-cuprous oxide shell nanozyme for surface-enhanced raman scattering and colorimetric
Xinge Ju1, Guantong Liu2, Xin Sun3
1College of Chemistry, Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, Jilin University, Qianjin Street 2669, Changchun 130012, PR China.
Abstract:
This work developed a dual-mode sensor based on aptamer regulation of silver core-cuprous oxide shell (Ag@Cu2O) nanozyme activity for kanamycin detection. The synthesized Ag@Cu2O nanoparticles served as both a surface-enhanced Raman scattering activity (SERS) substrate and a peroxidase-like catalytic activity. The aptamer adsorbed on the Ag@Cu2O surface enhanced the nanozyme activity through electrostatic interactions with positively charged 3,3',5,5'-tetramethylbenzidine (TMB). This promoted the oxidation of TMB to blue oxidized 3,3',5,5'-tetramethylbenzidine (ox-TMB) by hydrogen peroxide (H2O2). Upon kanamycin binding, the colorimetric absorbance and SERS intensity decreased. The SERS mode achieved a linear range of 0.01-1 μM and the limit of detection (LOD) of 1.78 nM. The colorimetric mode provided a linear range of 0.1-1 μM and a LOD of 47.7 nM. Good recoveries (92.48-102.57%) and low relative standard deviations (RSD < 5.0%) were achieved in spiked honey and mutton samples. This dual-mode design offered potential for food safety monitoring of kanamycin residues.
