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Unraveling SLAN+/- monocytes transcriptomics in lupus and extracellular vesicles effects
Paula X Losada1, Juan Antonio Villatoro-García2, Julio Jaramillo1
1Grupo de Inmunología Celular e Inmunogenética, Sede de Investigación Universitaria (SIU), Universidad de Antioquia, Medellín, Antioquia, Colombia.
Immunobiology
|January 17, 2026
Summary
Extracellular vesicles (EVs) from lupus nephritis (LN) patients promote inflammation and migration in monocytes. These findings implicate EVs in LN pathogenesis and suggest they are potential therapeutic targets for this kidney disease.
Area of Science:
- Immunology
- Nephrology
- Molecular Biology
Background:
- Lupus Nephritis (LN) is a severe complication of Systemic Lupus Erythematosus (SLE).
- Extracellular Vesicles (EVs) carrying autoantigens may form immune complexes that deposit in the kidney.
- SLAN marker identifies inflammatory, migratory monocytes relevant to LN.
Purpose of the Study:
- Analyze transcriptional profiles of circulating SLAN+/- monocytes in LN patients.
- Assess the impact of patient-derived EVs on SLAN- monocyte gene expression.
Main Methods:
- Isolated SLAN+/- monocytes from female LN patients and age-matched controls.
- Co-incubated plasma-derived EVs from LN patients with control SLAN- monocytes.
- Utilized next-generation RNA sequencing and bioinformatic analysis for gene expression profiling.
Main Results:
- LN patient monocytes showed elevated interferon response genes, indicating inflammation.
- SLAN+ monocytes exhibited pathways related to renal pathology, including stress, differentiation, and migration.
- EVs induced inflammatory responses and differentiation potential in non-SLAN monocytes.
Conclusions:
- EVs transport antigenic molecules, driving monocyte transcriptional changes towards inflammatory and migratory states.
- EVs are implicated in Lupus Nephritis pathogenesis.
- EVs represent potential therapeutic targets for LN treatment.
