Related Experiment Video

Updated: Jan 20, 2026

Differentiating Human-Induced Pluripotent Stem Cells into Brain Microvascular Endothelial Cells
03:15

Differentiating Human-Induced Pluripotent Stem Cells into Brain Microvascular Endothelial Cells

444

Protocol for morphogen-guided differentiation of brain cell types using human induced pluripotent stem cells

Lu Qian1, Juao-Guilherme Rosa2, Julia Tcw2

  • 1Department of Pharmacology, Physiology & Biophysics, Boston University Chobanian & Avedisian School of Medicine, Boston, MA 02118, USA.

STAR Protocols
|January 18, 2026
PubMed
Abstract

Keywords:
Cell cultureCell differentiationNeuroscienceStem cells

More Related Videos

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells07:37

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Published on: June 11, 2019

9.1K
Differentiating Human-Induced Pluripotent Stem Cells into Microglia-Like Cells04:03

Differentiating Human-Induced Pluripotent Stem Cells into Microglia-Like Cells

610

Related Concept Videos

Differentiating Human-Induced Pluripotent Stem Cells into Brain Microvascular Endothelial Cells03:15

Differentiating Human-Induced Pluripotent Stem Cells into Brain Microvascular Endothelial Cells

This video demonstrates a multi-step process for differentiating human iPSCs into brain microvascular endothelial cells. The resulting differentiated cells closely mimic brain microvascular endothelial cells, characterized by tight junctions, making them ideal for research on brain...
444
Differentiating Human-Induced Pluripotent Stem Cells into Microglia-Like Cells04:03

Differentiating Human-Induced Pluripotent Stem Cells into Microglia-Like Cells

This video demonstrates the procedure of the differentiation of human pluripotent cells into microglia-like cells. The process involves forming embryoid bodies, transferring them to coated wells, and using specific growth factors to guide differentiation into primitive macrophage precursors, which finally differentiate into microglia-like...
610
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells07:37

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like...
9.1K
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells06:40

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

We, based on knowledge from developmental biology and published research, developed an optimized protocol to efficiently generate A9 midbrain dopaminergic neurons from both human embryonic stem cells and human induced pluripotent stem cells, which would be useful for disease modeling and cell replacement therapy for Parkinson’s...
17.2K
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells10:24

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human...
22.0K
Differentiation of Human-Induced Pluripotent Stem Cells into Macrophages04:56

Differentiation of Human-Induced Pluripotent Stem Cells into Macrophages

This video describes an in vitro method for generating macrophages from human induced pluripotent stem cells (iPSCs). Macrophage generation from iPSCs occurs in three stages: (i) aggregation of iPSCs in suspension to form three-dimensional embryoid bodies (EBs) that differentiate into mesodermal cells, (ii) formation of macrophage precursor cells from the differentiated EBs, and (iii) differentiation of the macrophage precursor cells into mature, functional...
855