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Related Concept Videos

Reproducible Manufacturing of SPOT as a High-throughput Scaffold-based Culture Platform06:22

Reproducible Manufacturing of SPOT as a High-throughput Scaffold-based Culture Platform

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This protocol documents the fabrication and assembly of SPOT, a high-throughput scaffold-based culture platform, in 96- and 384-well formats. It also details the seeding process for SPOT, both manual and using a commercially available liquid...
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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics07:48

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A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line quality and high...
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This article details the manufacturing process for chimeric antigen receptor T cells for clinical use, specifically using an automated cell processor capable of performing viral transduction and cultivation of T cells. We provide recommendations and describe pitfalls that should be considered during the process development and implementation of an early-phase clinical...
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An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion08:29

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This protocol serves as a comprehensive tutorial for standardized and reproducible mixing of viscous materials with a novel open source automation technology. Detailed instructions are provided on the operation of a newly developed open source workstation, the usage of an open source protocol designer, and the validation and verification to identify reproducible...
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Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children06:22

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In this study, a method was developed to facilitate the transfer of experimental settings and analysis templates between two flow cytometers in two laboratories for the detection of lymphocytes in Japanese encephalitis-vaccinated children. The standardization method for the flow cytometer experiments will allow research projects to be effectively conducted in multiple...
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We have developed an automated cell culture and interrogation platform for micro-scale cell stimulation experiments. The platform offers simple, versatile, and precise control in cultivating and stimulating small populations of cells, and recovering lysates for molecular analyses. The platform is well suited to studies that use precious cells and/or...
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Related Experiment Video

Updated: Jan 20, 2026

Reproducible Manufacturing of SPOT as a High-throughput Scaffold-based Culture Platform
06:22

Reproducible Manufacturing of SPOT as a High-throughput Scaffold-based Culture Platform

Published on: July 29, 2025

886

Reproducible and Efficient Phenotyping for Cell Therapy Manufacturing Using the Accellix Automated Flow Cytometer

Jacob Van Vloten1, Seungmi Yoo2, Gurleen Sandhu2

  • 1Accellix, 2385 Bering Drive, San Jose, CA 95131; and.

PDA Journal of Pharmaceutical Science and Technology
|January 18, 2026
PubMed
Summary
This summary is machine-generated.

Automating cell therapy quality control with the Accellix Platform shows high repeatability and reproducibility. This advancement supports robust manufacturing for advanced cell therapies, ensuring safety and consistency.

Keywords:
AccellixCell therapyFlow cytometryGood Manufacturing PracticesTriumvira

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Last Updated: Jan 20, 2026

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Area of Science:

  • Cellular therapy
  • Biotechnology
  • Analytical chemistry

Background:

  • Cellular quality attributes are vital for ensuring the safety, efficacy, and consistency of cell therapies.
  • Current methods, like flow cytometry, face standardization challenges impacting regulatory compliance (Good Manufacturing Practice).
  • Critical Quality Attributes (CQAs) are essential for process monitoring and validating therapeutic effectiveness.

Purpose of the Study:

  • To evaluate the Accellix Platform for automated cellular sample analysis.
  • To assess the repeatability and reproducibility of the Accellix Platform across different sites, operators, and instruments.

Main Methods:

  • The Accellix Platform was used for automated cellular sample analysis.
  • Repeatability and reproducibility were assessed across multiple sites, operators, and dedicated instruments.

Main Results:

  • The Accellix Platform demonstrated feasibility for automated cellular sample analysis.
  • Data confirmed high repeatability and reproducibility of the platform across various testing conditions.

Conclusions:

  • The Accellix Platform shows potential for robust and compliant manufacturing of innovative cellular therapies.
  • Combining cytometry automation with advanced T cell engineering can enhance cell therapy production.