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The fate of the replisome following a collision with a head-on RNA polymerase (RNAP) is unknown. We find that the replisome stalls upon collision with a head-on RNAP, but resumes elongation after displacing the RNAP from DNA. Mfd promotes replication restart by facilitating displacement of the RNAP after the...
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Targeted cross-linking mass spectrometry creates quaternary protein structure models using mass spectrometry data acquired using up to three different acquisition protocols. When executed as a simplified workflow on the Cheetah-MS web server, the results are reported in a Jupyter Notebook. Here, we demonstrate the technical aspects of how the Jupyter Notebook can be extended for a more in-depth...
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Related Experiment Video

Updated: Jan 20, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
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Reply

Alexander Vanmaele1, Maria Karamanidou1, Petros Branidis1

  • 1Department of Cardiology, Thorax Centre, Cardiovascular Institute, Erasmus MC, Rotterdam, the Netherlands; Department of Vascular Surgery, Erasmus MC, Rotterdam, the Netherlands.

Journal of Vascular Surgery
|January 18, 2026
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Summary

No abstract available in PubMed .

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