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Related Concept Videos

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes07:26

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Related Experiment Video

Updated: Jan 20, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

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Dimerization-Enhanced Excimer Probe for Wash-Free Plasma Membrane Fluorescence Imaging.

Bailin Guo1, Enju Wang2, Yanling Liu1

  • 1Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province, College of Chemistry & Chemical Engineering, Hainan Normal University, Haikou, 571158, P. R. China.

Journal of Fluorescence
|January 18, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel membrane-targeting fluorescent probe (DIPP) that utilizes excimer formation for enhanced bioimaging. This probe offers bright red emission and rapid cellular uptake, making it ideal for advanced cellular imaging applications.

Keywords:
AmphiphilicityCell membraneExcimer emissionTriphenylimidazole dimerWash-free imaging

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Area of Science:

  • Chemical Biology
  • Biophysical Chemistry

Background:

  • Excimer probes offer advantages over monomeric fluorophores in bioimaging due to red-shifted spectra and larger Stokes shifts.
  • Developing targeted, sensitive fluorescent probes is crucial for advancing cellular imaging techniques.

Purpose of the Study:

  • To design and synthesize a novel excimer-forming, membrane-targeting fluorogenic probe (DIPP).
  • To evaluate the photophysical properties and bioimaging capabilities of DIPP.

Main Methods:

  • Covalent conjugation of two triphenylimidazole moieties via a 1,5-bis(pyridin-1-yl)pentane linker to create DIPP.
  • Spectroscopic analysis in various organic solvents and aqueous solutions with SDS micelles.
  • Cellular internalization and imaging studies to assess probe performance.

Main Results:

  • DIPP exhibited exclusive excimer fluorescence in most organic solvents, with dual emission in DMSO.
  • Negligible fluorescence in aqueous solution, with significantly enhanced excimer emission in SDS micelles.
  • DIPP demonstrated bright red emission (>610 nm), a large Stokes shift (>210 nm), rapid cellular uptake (~5 min), and low cytotoxicity.

Conclusions:

  • DIPP is an effective membrane-targeting fluorescent probe leveraging environmentally sensitive excimer formation.
  • The probe's properties are advantageous for wash-free, real-time bioimaging applications.
  • DIPP shows significant potential for advanced cellular and membrane imaging.