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Related Concept Videos

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer07:55

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We describe a method to utilize tumor-infiltrating lymphocytes (TILs) from mice through flow cytometry for adoptive cell transfer. This protocol aims to verify the specific cytotoxicity of TILs against tumors in a syngeneic pancreatic cancer mouse model, providing insights into the development of adoptive cell therapies for pancreatic...
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In the clinical context, patients with localized pancreatic cancer will undergo pancreatectomy followed by adjuvant treatment. This protocol reported here aims to establish a safe and effective method of modelling this clinical scenario in nude mice, through orthotopic implantation of pancreatic cancer followed by distal pancreatectomy and...
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Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is...
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Related Experiment Video

Updated: Jan 20, 2026

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer
07:55

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer

Published on: January 17, 2025

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TriCON: A Carbon-Based Triple-Modal Nanoplatform for Pancreatic Cancer Therapy.

Xinyu Peng1, Jiaxing Huang2, Shengnan Lv1

  • 1Department of Hepatobiliary and Pancreatic Surgery, General Surgery Center, The First Hospital of Jilin University, Changchun, P. R. China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|January 18, 2026
PubMed
Summary

A novel nanotherapy platform, TriCON, combines gene editing, chemotherapy, and immunotherapy to overcome pancreatic cancer's resistance. This approach reprograms the tumor microenvironment, enhancing treatment efficacy and promoting tumor regression.

Keywords:
TriCONchemo‐immunotherapygene editingpancreatic cancerpoliovirus receptor

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An Orthotopic Resectional Mouse Model of Pancreatic Cancer
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Last Updated: Jan 20, 2026

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer
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An Orthotopic Resectional Mouse Model of Pancreatic Cancer
07:17

An Orthotopic Resectional Mouse Model of Pancreatic Cancer

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Area of Science:

  • Oncology
  • Nanotechnology
  • Gene Editing

Background:

  • Pancreatic cancer exhibits high malignancy and a tumor-suppressive microenvironment, limiting conventional treatments.
  • Reprogramming the tumor microenvironment is crucial for enhancing cancer immunotherapy effectiveness.
  • CRISPR/Cas9 gene editing offers precise control over immunosuppression-related gene expression, but delivery vectors face challenges.

Purpose of the Study:

  • To develop and evaluate a novel triple-modality therapeutic platform (TriCON) for pancreatic ductal adenocarcinoma (PDAC).
  • To synergize gene editing, chemotherapy, and immunotherapy for enhanced antitumor activity.
  • To address limitations in current gene editing delivery systems.

Main Methods:

  • Development of TriCON, a nanotherapy platform integrating CRISPR/Cas9 gene editing (targeting poliovirus receptor), doxorubicin chemotherapy, and checkpoint blockade immunotherapy.
  • Evaluation of TriCON's spatiotemporal convergence, stimuli-responsive controllability, and tumor-microenvironment modulatory conductivity.
  • Assessment of in vivo gene editing efficiency, tumor regression, and immune cell activation in PDAC models.

Main Results:

  • TriCON demonstrated synergistic antitumor activity in PDAC models, leading to tumor regression.
  • The platform enhanced chemotherapy efficacy, induced immunogenic cell death, and activated natural killer (NK) cells.
  • Achieved 14.2% PVR gene editing efficiency in vivo through optimized endosomal escape and CRISPR release.

Conclusions:

  • TriCON establishes a programmable nanotherapeutic paradigm for pancreatic cancer.
  • The platform effectively synergizes gene editing precision with chemo-immunotherapy.
  • This approach offers a novel framework for treating pancreatic ductal adenocarcinoma by reprogramming the tumor microenvironment.