Recent Advances in Enzyme Activity Assay Methods: A Systematic Review of Emerging Technologies and Applications

Enzyme kinetics describes the catalytic effects of enzymes, which are biomolecules that facilitate chemical reactions necessary for living organisms. Enzymes act on molecules, referred to as substrates, to form products. Enzyme kinetic parameters are determined via assays that directly or indirectly measure changes in substrate or product concentration over time. 
This video will cover the basic principles of enzyme kinetics (including rate equations) and kinetic models. The concepts...

In this video, we demonstrate a high throughput assay for carbohydrate-active enzyme screening using insoluble chromogenic polymer hydrogel substrates. The enzyme degrades these substrates into a soluble colored product that can be quantified by measuring the color intensity of the...

Investigating the Effect of pH and Temperature on Peroxidase Activity
ExpandTo make the extraction buffer for the peroxidase enzyme, mix equal volumes of 0.1 M sodium phosphate monobasic and 0.1 molar sodium phosphate dibasic solutions to achieve a final volume of 500 mL.
Test the resulting solution using a pH meter. The mixture should be between a pH of 6.8 and 7.2.
To extract the turnip peroxidase, use a knife to remove the outer layer of a turnip and then cut the turnip into approximately...

Biological Catalysts

Baseline Peroxidase Enzyme Experiment
ExpandTo begin, the baseline for the peroxidase enzyme reaction must be determined. Make the substrate by adding 7 mL of distilled water to a clean test tube and then add 0.2 mL of guaiacol. Note: Guaiacol is a color-changing indicator that becomes more yellow-orange as the enzyme reaction progresses.
Next, add 0.3 mL of 0.1% H2O2.
Using a marker, label the test tube substrate and then cover the tube with a piece of sealing film.
Holding the cover in place,...

Detailed herein are the operation and assembly protocols of a modular microfluidic screening platform for the systematic characterization of colloidal semiconductor nanocrystal syntheses. Through fully adjustable system arrangements, highly efficient spectra collection may be carried out across 4 orders of magnitude reaction time scales within a mass transfer-controlled sampling...