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Updated: Jan 20, 2026

Fundus Photography as a Convenient Tool to Study Microvascular Responses to Cardiovascular Disease Risk Factors in Epidemiological Studies
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Increased mROS Generation Associates With Cardiovascular Risk in BioHEART-CT PBMCs.

W Eugene Lee1,2, Albert Henry3, Eleanor Ruth Spenceley3,4

  • 1Faculty of Medicine and Health, University of Sydney, Sydney, New South Wales, Australia.

Clinical and Translational Science
|January 19, 2026
PubMed
Summary
This summary is machine-generated.

Mitochondrial reactive oxygen species (mROS) in blood cells are not reliable biomarkers for coronary artery disease (CAD). Further research is needed to identify effective blood-based markers for cardiovascular disease.

Keywords:
PBMCsatherosclerosisbiomarkercoronary artery diseasedysfunctionmitochondria

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Area of Science:

  • Cardiovascular Research
  • Biomarker Discovery
  • Immunology

Background:

  • Coronary artery disease (CAD) is a major global health concern, necessitating the identification of accessible blood biomarkers.
  • Oxidative stress and immune cell dysfunction are implicated in atherosclerosis, the underlying pathology of CAD.

Purpose of the Study:

  • To investigate the association between mitochondrial reactive oxygen species (mROS) production in peripheral blood mononuclear cells (PBMCs) and CAD.
  • To explore the differential expression of the CCBE1 gene in PBMC populations in relation to CAD.

Main Methods:

  • Analysis of PBMCs from 40 participants with or without CT-defined CAD using MitoSOX-based flow cytometry to measure mROS.
  • Single-cell RNA sequencing (scRNA-seq) to assess CCBE1 gene expression across PBMC subtypes.

Main Results:

  • PBMC mROS levels did not show consistent associations with CAD status or cardiovascular risk factors.
  • Exploratory subgroup analyses revealed limited, non-uniform signals for lymphocyte and monocyte mROS.
  • scRNA-seq did not identify a distinct CCBE1 expression signature in PBMCs.

Conclusions:

  • PBMC-derived mROS is unlikely to be a useful cross-sectional biomarker for CAD in stable populations.
  • Current findings do not support the use of mROS in PBMCs as a general biomarker for coronary artery disease.