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Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface10:38

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory...
38.1K
Isolating Nasal Olfactory Stem Cells from Rodents or Humans09:19

Isolating Nasal Olfactory Stem Cells from Rodents or Humans

We describe here a method for biopsying olfactory mucosa from rat and human nasal cavities. These biopsies can be used for either identifying molecular anomalies in brain diseases or isolating multipotent adult stem cells that can be utilized for cell transplantation in animal models of brain...
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Nasal Wipes for Influenza A Virus Detection and Isolation from Swine05:59

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

The authors present a protocol to collect swine nasal wipes to detect and isolate influenza A viruses.
9.8K
Murine Nasal Lavage Fluid Collection without Blood Contamination05:12

Murine Nasal Lavage Fluid Collection without Blood Contamination

The present protocol describes a novel method for avoiding blood contamination in murine nasal lavage fluid (NLF). Since this method ensures the NLF collection without blood contamination, it allows for more accurate detections of immunological components and various respiratory...
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Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment04:35

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

Saline nasal lavage can be used to sample the canine nasal immune microenvironment. Because the approach is relatively non-invasive and does not disrupt the nasal tissues, it can be performed serially. Cells and proteins collected from the nasal lavage technique can be processed for various laboratory analyses.
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Nose and Nasal Cavity01:24

Nose and Nasal Cavity

The nose is composed of an observable exterior segment (external nose) and an internal segment within the skull known as the nasal cavity (internal nose). The external nose, visible on the face, consists of a framework of bone and hyaline cartilage enveloped in skin and muscle and lined with a mucous membrane. This structure is supported by the frontal bone, nasal bones, and maxillary bone and is supplemented by a cartilaginous framework comprising the septal nasal cartilage, lateral nasal...
10.7K