CircDYRK1A Suppresses Gastric Cancer Progression via Sponging miR-331-5p to Regulate GADD45A

Numerous studies have demonstrated that circRNAs exhibit abnormal expression patterns in cancer and contribute to the regulation of malignant tumor progression. This study aims to investigate the role of circDYRK1A in the progression of Gastric Cancer (GC). Bioinformatics and qRT-PCR analyses revealed significant downregulation of circDYRK1A in GC tissues and cell lines. The circular structure and stability of circDYRK1A were confirmed via Sanger sequencing, electrophoresis, and RNase R treatment. Subcellular localization experiments indicated predominant cytoplasmic distribution. The effects of circDYRK1A on the functions of GC cells were evaluated through CCK-8, EDU proliferation and Transwell assays. The interaction between circDYRK1A and miR-331-5p/GADD45A was validated by dual luciferase assay, RNA immunoprecipitation, western blotting, and rescue assay. The research results showed that circDYRK1A was significantly down-regulated in GC and inhibited the proliferation, migration and invasion of GC cells by the miR-331-5p/GADD45A axis. This study lays a theoretical foundation for the molecular mechanism by which circDYRK1A inhibits the progression of GC and opens up new potential therapeutic approaches for targeted treatment of GC.