The genome sequence of the Barred Straw, Gandaritis pyraliata (Denis & Schiffermüller, 1775)

Long-read sequences greatly facilitate the assembly of complex genomes and characterization of structural variation. We describe a method to generate ultra-long sequences by nanopore-based sequencing platforms. The approach adopts an optimized DNA extraction followed by modified library preparations to generate hundreds of kilobase reads with moderate coverage from human...

The purpose of this protocol is to use a combination of computational and bench research to find novel sequences that cannot be easily separated from a co-purifying sequence, which may be only partially...

We present a public computational web site for the analysis of genomic sequences. It detects DNA sequence patterns with various non-random nucleotide compositions. This resource also generates randomized sequences with diverse levels of...

We describe a protocol for amplifying retroviral integration sites from the genomic DNA of infected cells, sequencing the amplified virus-host junctions, and then mapping these sequences to a reference genome. We also describe techniques to quantify the distribution of integration sites relative to various genomic annotations using...

A bar graph is also called a bar chart and consists of bars that are separated from each other. It either uses horizontal or vertical bars to show comparisons among categories. The bars can be rectangles, or they can be rectangular boxes (used in three-dimensional plots). One axis of the graph represents the specific categories being compared, and the other axis shows a discrete value. In this graph, the length of the bar for each category is proportional to the number or percent of individuals...

We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow...