Ginsenoside Rb1 restores dental pulp by promoting odontogenic differentiation of dental pulp stem cells via the
Ok Hyung Nam1,2, Seong Eun Kim3, Si Won Kang3
1Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
Background:
Dental surgery is a common treatment for pulpitis, which can lead to decreased pulp vitality. A novel approach is needed to induce reparative dentin formation without pulp removal. Ginsenoside Rb1 (G-Rb1) is known for its regenerative properties in various diseases. This study aimed to examine the odontogenic differentiation-promoting effects of G-Rb1 in human dental pulp stem cells (hDPSCs) and a rat pulpotomy model, and explore the underlying molecular mechanisms.
Methods:
hDPSCs were extracted from supernumerary teeth and cultured with 10 μg/mL G-Rb1. Odontoblastic differentiation was assessed through real-time PCR, western blot, ALP activity, and Alizarin Red S staining. RNA sequencing was performed to confirm the effects of G-Rb1 on odontogenesis of hDPSCs. The effects in the rat pulpotomy model were evaluated by examining reparative dentin formation using von Kossa staining.
Results:
G-Rb1 was non-cytotoxic and significantly enhanced hDPSC migration and odontoblastic differentiation in hDPSCs, upregulating the expression of odontogenic markers, DMP1 and DSPP, and increasing mineralized nodule deposition. RNA sequencing identified the TGF-β/Smad pathway as a key mechanism underlying these effects, which was confirmed by evaluating the expression of pathway-related proteins. In vivo analysis demonstrated significantly higher reparative dentin formation in the G-Rb1-treated group compared to the control group.
Conclusion:
G-Rb1 promotes odontoblastic differentiation in hDPSCs and enhances reparative dentin formation via the TGF-β/Smad signaling pathway. These findings highlight the potential of G-Rb1 as a therapeutic agent for dental pulp repair and regeneration.
Related Concept Videos
14:52Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
03:45Primary Culture of Dental Pulp Stem Cells
07:32In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
11:50Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
08:06A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
02:33Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth