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RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide...

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

A highly sensitive ribozyme-based assay, applicable to high-throughput screening of chemicals targeting the unique process of RNA editing in trypanosomatid pathogens, is described in this paper. Inhibitors can be used as tools for hypothesis-driven analysis of the RNA editing process and ultimately as...

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

A significant barrier to technologies like CRISPR is the off-target events that can disrupt vital genes. 'Circularization for In Vitro Reporting of Cleavage Effects by Sequencing' (CIRCLE-seq) is a technique designed to identify unintended cleavage sites. This method maps the genome-wide activity of CRISPR-Cas9 with high sensitivity and without...

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the...

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Related Experiment Video

Updated: Jan 20, 2026

RNA Editing: Post-transcriptional Base Insertion, Deletion or Modification

RNA Editing: Post-transcriptional Base Insertion, Deletion or Modification

Targeted RNA base editing for therapeutic: mechanisms and advances.

Weikai Yan¹, Xiaocheng Weng¹

¹College of Chemistry and Molecular Sciences, State Key Laboratory of Metabolism and Regulation in Complex Organisms, Department of Otorhinolaryngology-Head and Neck Surgery, Zhongnan Hospital of Wuhan University, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430072, China.

Pharmaceutical Science Advances

|January 19, 2026

Summary

RNA base editing offers precise correction of genetic mutations by modifying RNA molecules. This review details RNA editing systems, types, and therapeutic applications for genetic diseases.

Keywords:

CRISPR-Cas Point mutation RNA editing RNA modification

More Related Videos

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

Related Experiment Videos

Last Updated: Jan 20, 2026

RNA Editing: Post-transcriptional Base Insertion, Deletion or Modification

RNA Editing: Post-transcriptional Base Insertion, Deletion or Modification

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

Area of Science:

Molecular Biology
Genetic Engineering
Biotechnology

Background:

RNA base editing is a rapidly advancing technology for correcting disease-associated point mutations.
It offers an alternative to CRISPR-based genome editing with increasing efficiency and precision.

Purpose of the Study:

To review the core components of RNA base editing systems.
To describe major RNA editing types and their research progress.
To summarize delivery methods and therapeutic applications for genetic diseases.

Main Methods:

Review of RNA-targeting systems and effector proteins.
Analysis of major RNA editing types (A-to-I, C-to-U, A-to-m6A/m6A-to-A, U-to-Ψ).
Systematic summary of delivery methods and disease treatment explorations.

Main Results:

Detailed overview of RNA base editing system components and types.
Compilation of research progress for various RNA editors.
Summary of delivery strategies and initial therapeutic applications for nonsense mutations.

Conclusions:

RNA base editing is a powerful tool with significant potential for treating genetic disorders.
Current challenges and future directions in RNA editing technology are discussed.