Cutaneous malignancies mimicking scars: A case series highlighting pearls and pitfalls

This protocol describes the use of macromolecular crowding to create an in vitro human hypertrophic scar tissue model that resembles in vivo conditions. When cultivated in a crowded macromolecular environment, human skin fibroblasts exhibit phenotypes, biochemistry, physiology, and functional characteristics resembling scar...

This protocol describes the generation of a skin-fascia explant termed "SCar like tissue in A Dish" or SCAD. This model allows unprecedented visualization of single fibroblasts during scar...

Transgenic lck:eGFP zebrafish express GFP highly in T lymphocytes, and have been used to study T cell development and acute lymphoblastic leukemia. This line can be used to study B cells, which express lck at lower levels. This protocol describes purification of malignant and non-malignant B cells from lck:eGFP...

A demonstration of the bedside test for cutaneous allodynia and its clinical...

This protocol will explain how to establish a hypertrophic scarring murine model that increases mechanotransduction signaling to simulate human-like scarring. This method involves increasing mechanical tension across a healing incision in a mouse and using a specialized device to create reproducible, excessive scar tissue for detailed histological and bioinformatic...

Leasing offers businesses a strategic method to access assets without significant upfront investment, making it a popular choice for managing operational and financial flexibility. However, this approach can also present challenges businesses must carefully navigate to avoid adverse outcomes.One of the primary considerations in leasing is the potential for higher costs over the long term. While leasing can spread payments over time and reduce immediate financial burdens, the aggregate payments...