Mitochondrial genome dataset of Drosophila melanogaster Meigen, 1830 (fruit fly) from Pakistan

Drosophila melanogaster is one of the most employed animal models and was amongst the first organisms selected for genome sequencing. Geographically isolated populations of D. melanogaster have shown to harbour distinct allelic makeup. In this data article, we present the whole mitochondrial draft genome sequence of a white eye mutant of D. melanogaster, dubbed as ANU-1. The strain was isolated from wildtype population. This is the first report of the mitochondrial genome of D. melanogaster from Pakistan. The complete mitochondrial genome of D. melanogaster ANU-1 is 19,116 bp in length containing 13 protein coding genes, 22 tRNA genes, 2 rRNA genes and an AT-rich region of 4208 bp in length. Phylogenetic analysis based on mitochondrial DNA sequences clustered D. melanogaster ANU-1 with other species of melanogaster subgroup. Genome sequence comparison identified novel alleles in COX1, ND5 and CYTB genes in D. melanogaster ANU-1 with subtle structural variations in the encoded proteins. The present mitochondrial genome provides the first glimpse of allelic variations in D. melanogaster from Pakistan and was deposited in GenBank under the accession number OR555819.1.