Related Experiment Video
Updated: Jan 20, 2026
08:12
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
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Long non-coding RNA POC1B-AS1 depletion represses colorectal cancer progression through the microRNA-625-5p/FOXK1
Hairui Liu1, Xueying Zhang2, Xianmei Jin3
1Department of Abdominal Surgery, Jilin Cancer Hospital Changchun 130021, Jilin, China.
American Journal of Translational Research
|January 19, 2026
Summary
POC1B-AS1 promotes colorectal cancer (CRC) aggressiveness by sponging miR-625-5p and upregulating FOXK1. Targeting this pathway offers a potential therapeutic strategy for CRC treatment.
Area of Science:
- Oncology
- Molecular Biology
- RNA Biology
Background:
- Aberrant expression of long non-coding RNAs (lncRNAs) contributes to colorectal cancer (CRC) aggressiveness.
- Identifying novel lncRNAs involved in CRC progression is crucial for developing therapeutic targets.
Purpose of the Study:
- To investigate the role of POC1B antisense RNA 1 (POC1B-AS1) in colorectal cancer (CRC) malignancy.
- To elucidate the molecular mechanism underlying POC1B-AS1's function in CRC.
Main Methods:
- Quantitative real-time polymerase chain reaction (qRT-PCR) for POC1B-AS1 expression analysis.
- In vitro and in vivo functional assays to assess the biological roles of POC1B-AS1.
- Luciferase reporter and RNA immunoprecipitation (RIP) assays to determine molecular interactions.
Main Results:
- POC1B-AS1 was highly expressed in CRC tissues.
- POC1B-AS1 depletion reduced CRC cell proliferation, colony formation, migration, and invasion in vitro, and suppressed tumor growth in vivo.
- POC1B-AS1 acted as a molecular sponge for microRNA-625-5p (miR-625-5p), leading to the overexpression of forkhead box K1 (FOXK1) in CRC. The effects of POC1B-AS1 ablation were reversed by miR-625-5p downregulation or FOXK1 overexpression.
Conclusions:
- POC1B-AS1 promotes CRC malignancy by sequestering miR-625-5p and upregulating FOXK1.
- The POC1B-AS1/miR-625-5p/FOXK1 pathway represents a potential therapeutic target for CRC.
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