A User-Centered Approach in the Development of a Label-Free Impedimetric Chemiresistor for H₂O₂

Area of Science:

• Biomedical Engineering
• Materials Science
• Analytical Chemistry

Background:

• Laboratory optimization in sensing technology often hinders real-world application.
• A user-centered approach is crucial for translating lab prototypes into healthcare solutions.

Purpose of the Study:

• To develop a novel electrochemical sensing modality for a noninvasive, point-of-care device.
• To demonstrate a proof-of-concept impedimetric chemiresistor (ICR) for potential diagnostic use.

Main Methods:

• Fabrication of a two-electrode ICR using low-cost, scalable roll-to-roll (R2R) flexographic printing.
• Incorporation of a zeolitic imidazolate framework-8 (ZIF-8) chemiresistive channel.
• Testing the ICR's response to hydrogen peroxide (H2O2) and validating in human saliva.

Main Results:

• The ICR demonstrated a linear impedance response to hydrogen peroxide (H2O2).
• Achieved a limit-of-detection of 3.7 mM and a dynamic range of 4-60 mM for H2O2.
• Preliminary validation in human saliva confirmed device feasibility.

Conclusions:

• The developed ICR, utilizing R2R printing and ZIF-8, offers a promising platform for noninvasive diagnostics.
• User feedback integration supports the transition of sensing technology from lab to real-world healthcare.
• This work represents an initial step towards a noninvasive hypoglycemia diagnostic tool.