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Updated: Jan 20, 2026

Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
Published on: August 29, 2017
Generation of Low-Cost User-Customizable Neutral Density Filters and the Involvement of Undergraduate Researchers
Julia Filip1, Joseph Eppich1, Melissa Y Gallardo1
1Department of Chemistry and Physics, Florida Gulf Coast University, 10501 FGCU Blvd South, Fort Myers, Florida 33965-6565, United States.
Abstract:
In research and teaching laboratories, optical attenuation of a light source is often required on short notice, yet access to suitable filters may be limited, resulting in delays or increased expense. For monochromatic applications, attenuation can be achieved using individual solutions of absorptive dyes. However, broadband or spectral work typically necessitates a suite of neutral density (ND) filters covering the desired wavelength range and attenuation levels. This work presents a low-cost, customizable approach for formulating liquid ND filters from common laboratory dyes. Two scenarios are considered: laboratories sourcing dyes for purchase and those leveraging existing dye inventories. An example ND solution prepared from in-house materials rivals commercial ND filters: from 425 to 575 nm, abs. range 2.04-2.16 (0.7-0.9%T), absavg = 2.09 (0.8%T), and s.d.abs = 0.03. This stock solution can be diluted as needed and is compatible with a range of optical systems (e.g., OPO lasers, fluorescence setups). The formulation strategy is generalizable to other dye sets and application requirements.
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