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Related Concept Videos

Controlled Cortical Impact Model for Traumatic Brain Injury05:30

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This video demonstrates the delivery of Traumatic Brain Injury (TBI) in mice. After exposing the skull, the TBI site is marked. The mouse is then positioned under the TBI device. The impact is delivered by lifting the metal rod and allowing it to drop freely onto the impactor, which is in contact with the...
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Related Experiment Video

Updated: Jan 20, 2026

Controlled Cortical Impact Model for Traumatic Brain Injury
05:30

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Predictive N‑Glycan Signatures of Severe Traumatic Brain Injury in Biofluids Using LC-MS/MS.

Joy Solomon1, Sherifdeen Onigbinde1, Moyinoluwa Adeniyi1

  • 1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, United States.

ACS Omega
|January 19, 2026
PubMed
Summary

Severe traumatic brain injury (sTBI) alters N-glycan patterns in serum and cerebrospinal fluid (CSF). These changes, particularly in fucosylation and sialylation, show potential as biomarkers for tracking TBI progression and improving patient prognosis.

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Related Experiment Videos

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Biomarker Discovery

Background:

  • Traumatic brain injury (TBI) is a major cause of long-term neurological deficits.
  • Accurate diagnosis and prognosis of TBI remain challenging despite advances in biomarker research.
  • Glycosylation's role in TBI pathogenesis is not well understood, yet it's crucial for neuronal function and neuroinflammation.

Purpose of the Study:

  • To investigate alterations in N-glycosylation patterns in serum and CSF following severe TBI (sTBI).
  • To identify potential glycan-based biomarkers for monitoring TBI progression and prognosis.

Main Methods:

  • Analysis of N-glycans in serum and CSF from sTBI patients at multiple time points post-injury.
  • Utilized advanced glycomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
  • Identified and quantified N-glycans, including isomeric structures.

Main Results:

  • Identified 102 N-glycans in serum and 86 N-glycans in CSF.
  • Observed significant alterations in N-glycan expression, including differential fucosylation (increased in serum, decreased in CSF) and sialylation.
  • Identified specific N-glycan isomers with potential as biomarkers for TBI progression.

Conclusions:

  • Glycomic profiles in serum and CSF change significantly after sTBI, reflecting systemic and central nervous system responses.
  • Altered glycosylation patterns suggest involvement in neuroinflammation and neurodegeneration.
  • N-glycans represent promising biomarkers for tracking TBI progression and could inform therapeutic strategies and prognosis.