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Related Experiment Video

Updated: Jan 20, 2026

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A Pilot Study Profiling Aqueous Humor-Derived Exosomal MicroRNA in Primary Open-Angle and Exfoliation Glaucoma.

Yunyun Zou1, Jaeryung Kim1, Taeun Kim1

  • 1Department of Ophthalmology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.

Ophthalmology Science
|January 19, 2026
PubMed
Summary

This study profiles aqueous humor exosomal microRNAs (miRNAs) in primary open-angle glaucoma (POAG) and exfoliation glaucoma (XFG). Downregulated miR-29a-3p suggests its role in extracellular matrix remodeling and potential as a glaucoma biomarker.

Keywords:
Aqueous humor–derived exosomesExfoliation glaucomaGlaucomaMicroRNA sequencingPrimary open-angle glaucoma

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Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Biomarker Discovery

Background:

  • Glaucoma pathogenesis involves extracellular matrix (ECM) remodeling.
  • Exosomal microRNAs (miRNAs) in aqueous humor (AH) are potential biomarkers.
  • Primary open-angle glaucoma (POAG) and exfoliation glaucoma (XFG) are major forms of glaucoma.

Purpose of the Study:

  • To profile AH-derived exosomal miRNAs in POAG and XFG.
  • To investigate their potential as diagnostic biomarkers.
  • To explore their role in ECM remodeling in glaucoma pathogenesis.

Main Methods:

  • Prospective comparative study of AH from POAG, XFG patients, and healthy donors.
  • Small RNA sequencing of exosomes isolated from AH.
  • Functional validation of miR-29a in human trabecular meshwork cells (HTMCs) models.

Main Results:

  • Identified 130 and 145 differentially expressed miRNAs in POAG/Healthy and XFG/Healthy comparisons.
  • Discovered a glaucoma-specific signature of upregulated ECM-related miRNAs.
  • Found miR-29a-3p significantly downregulated in glaucoma and attenuated collagen deposition in HTMCs.

Conclusions:

  • First profile of AH-derived exosomal miRNAs in POAG and XFG.
  • Identified potential miRNAs linked to ECM remodeling.
  • Highlighted utility of these miRNAs as glaucoma biomarkers and therapeutic targets.