Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Preparation of High-Temperature Sample Grids for Cryo-EM05:05

Preparation of High-Temperature Sample Grids for Cryo-EM

4.2K
This paper provides a detailed protocol for preparing sample grids at temperatures as high as 70 °C, prior to plunge freezing for cryo-EM...
4.2K
Cryo-EM and Single-Particle Analysis with Scipion09:06

Cryo-EM and Single-Particle Analysis with Scipion

4.4K
Single-particle analysis in cryo-electron microscopy is one of the main techniques used to determine the structure of biological ensembles at high resolution. Scipion provides the tools to create the whole pipeline to process the information acquired by the microscope and achieve a 3D reconstruction of the biological...
4.4K
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion13:43

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

15.1K
This article describes how to effectively utilize three cryo-EM processing platforms, i.e., cryoSPARC v3, RELION-3, and Scipion 3, to create a single and robust workflow applicable to a variety of single-particle data sets for high-resolution structure...
15.1K
Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon04:52

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

2.7K
The present protocol describes a generalized and easy-to-implement scheme for tilted single-particle data collection in cryo-EM experiments. Such a procedure is especially useful for obtaining a high-quality EM map for samples suffering from preferential orientation bias due to adherence to the air-water...
2.7K
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

5.9K
High-resolution cryo-EM maps of macromolecules can be also achieved by using 200 kV TEM microscopes. This protocol shows the best practices for setting accurate optics alignments, data acquisition schemes, and selection of imaging areas that are all essential for the successful collection of high-resolution datasets using a 200 kV TEM.
5.9K
The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples07:27

The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

2.7K
The peel-blot technique is a cryo-EM grid preparation method that allows for the separation of multilayered and concentrated biological samples into single layers to reduce thickness, increase sample concentration, and facilitate image...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Universal parameters of bulk-solvent masks.

Acta crystallographica. Section A, Foundations and advances·2024
Same author

Efficient structure-factor modeling for crystals with multiple components.

Acta crystallographica. Section A, Foundations and advances·2023
Same author

Direct calculation of cryo-EM and crystallographic model maps for real-space refinement.

Acta crystallographica. Section D, Structural biology·2022
Same author

Analytic modeling of inhomogeneous-resolution maps in cryo-electron microscopy and crystallography.

IUCrJ·2022

Related Experiment Video

Updated: Jan 20, 2026

Preparation of High-Temperature Sample Grids for Cryo-EM
05:05

Preparation of High-Temperature Sample Grids for Cryo-EM

Published on: July 26, 2021

4.2K

Quantifying distributions of cryo-EM projections.

Alexandre G Urzhumtsev1

  • 1Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC (Institute of Genetics and of Molecular and Cellular Biology), Centre National de la Recherche Scientifique (CNRS) UMR 7104/Institut National de la Santé de la Recherche Médicale (Inserm) U964/Université de Strasbourg, 1 Rue Laurent Fries, 67404 Illkirch, France.

Acta Crystallographica. Section D, Structural Biology
|January 19, 2026
PubMed
Summary

This study introduces a new quantitative method to assess the uniformity of particle views in cryo-electron microscopy (cryo-EM). This approach simplifies data analysis and storage for cryo-EM datasets, improving model building.

Keywords:
angular distribution of viewscryo-EMfrequency distribution curvenon-uniform distributionquantitative distribution measures

More Related Videos

Cryo-EM and Single-Particle Analysis with Scipion
09:06

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

4.4K
A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

15.1K

Related Experiment Videos

Last Updated: Jan 20, 2026

Preparation of High-Temperature Sample Grids for Cryo-EM
05:05

Preparation of High-Temperature Sample Grids for Cryo-EM

Published on: July 26, 2021

4.2K
Cryo-EM and Single-Particle Analysis with Scipion
09:06

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

4.4K
A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

15.1K

Area of Science:

  • Structural biology
  • Biophysics
  • Computational imaging

Background:

  • Non-uniform angular distributions of particle views in cryo-electron microscopy (cryo-EM) complicate image processing and model building.
  • Traditional 2D diagrams for representing view distributions are prone to distortions and lack quantitative uniformity measures.
  • Current methods for storing and comparing view distributions are cumbersome and lack standardization.

Purpose of the Study:

  • To develop a quantitative method for characterizing angular distributions of particle views in cryo-EM.
  • To replace cumbersome 2D diagrams with a more efficient and standardized representation.
  • To facilitate improved data management and analysis in cryo-EM.

Main Methods:

  • Proposing a novel method to quantitatively characterize angular distributions of particle views.
  • Representing these distributions as 1D curves instead of 2D colored diagrams.
  • Developing quantitative measures of uniformity for cryo-EM datasets.

Main Results:

  • A new method for quantitative characterization of angular distributions has been developed.
  • The proposed method represents view distributions as simple 1D curves.
  • This approach offers a standardized and compact description of angular distributions.

Conclusions:

  • The proposed quantitative measures provide a standardized and compact description of angular distributions.
  • This method can be integrated into databases like EMDB for improved data management.
  • The new representation simplifies cryo-EM data analysis and comparison across datasets.