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Interslice Magnetization Transfer Ratio Imaging for Meningioma Detection01:26

Interslice Magnetization Transfer Ratio Imaging for Meningioma Detection

Begin with a patient with meningioma, or a brain tumor, positioned in an MRI scanner for non-invasive brain imaging.Adjust the scanner to obtain structural images of individual slices of the brain.Enhance the contrast to improve tissue differentiation.Use interslice magnetization transfer ratio imaging on brain slices using radiofrequency pulses slightly offset from the MRI signal.The pulses deliver energy to the proton pool in macromolecules and bound water molecules.This energy transfers to...
176
Concurrent Quantification of Cellular and Extracellular Components of Biofilms10:18

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

A protocol for the concurrent quantification and comparison of three cellular and extracellular components within biofilms is presented. The methodology involves the use of confocal laser scanning microscopy, biofilm structural analysis and visualization software, and statistical analysis...
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Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit06:11

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit

Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in...
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Multianalyte Immunobead Assay: A Flow-Based Immunoassay Technique for Concurrent Detection of Multiple Antibody Subtypes in Human Serum Sample04:45

Multianalyte Immunobead Assay: A Flow-Based Immunoassay Technique for Concurrent Detection of Multiple Antibody Subtypes in Human Serum Sample

This video describes an immunoassay technique using fluorescent magnetic beads or microspheres coupled with different antigens to simultaneously detect various antibody isotypes in a serum sample. The assay is helpful in understanding seroconversion during an infection or...
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Porcine Model of Infrarenal Abdominal Aortic Aneurysm11:13

Porcine Model of Infrarenal Abdominal Aortic Aneurysm

This novel model creates robust infrarenal abdominal aortic aneurysms in swine using a combination of balloon angioplasty, elastase/collagenase perfusion, topical elastase application, and oral compound β-aminopropionitrile administration, which interferes with collagen...
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Intracranial Pharmacotherapy and Pain Assays in Rodents02:26

Intracranial Pharmacotherapy and Pain Assays in Rodents

Here we present a protocol to perform intracranial pharmacological experiments followed by pain behavior assays in rodents. This protocol allows researchers to deliver molecular and cellular targets in the brain, for pharmacologic agents in the treatment of...
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